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1) Oxygen Diffusers are awful. Algae Terrariums are superior if under light. Leave the polluted items they drop in the room with them and use Oxidizers to clean it up and it clears up that CO2 buildup early on.
2) In your first 30 cycles the food produced by wild plants is generally enough. No need for a farm. When building, don't dig them up and just set them to auto-harvest. Added bonus is making a Sanctuary out of it for +6 Morale! Plus there's no need for Microbe Musher at least on Terra.
3)Stagger your dupes' schedule so that they don't have the same Downtime period and thus you only need 1 MAYBE 2 bathrooms for 10+ dupes.
4) Toilets and Showers can use water fresh out of the sieve, but the sinks need freshwater. But due to urine the water in that loop will always build up so once you get started it'll feed itself. Save excess POLLUTED water in that loop. You'll need a lot of Polluted water in-game.